Attività di Ricerca 

Gli studi del mio laboratorio vertono su tematiche ed analisi di biologia molecolare ed epigenetica applicate al miglioramento della conoscenza della patogenesi ed identificazione di trattamenti delle malattie neuromuscolari, con una particolare attenzione alle distrofie muscolari ed alle forme di atrofia muscolare secondarie ad immobilizzazione, denervazione ed invecchiamento. A tal fine utilizziamo modelli di culture di linee cellulari, cellule primarie isolate da modelli murini di malattia e cellule da pazienti affetti da malattie di interesse (incluse "induced pluripotent cells" convertite in muscolo e cellule isolate da biopsie. A tal riguardo, il nostro lavoro e' sempre piu' concetrato sul passaggio da modelli pre-clinici di malattia all'analisi di cellule derivate dai pazienti, al fine di generare informazioni e scoperte scientifiche applicabili a patologie umane. In quest'ambito svolgiamo collaborazioni con laboratori esteri (in particolare con il SanfordBurnhamPrebys Medical Discrovery Institute in San Diego) ed industrie interessate a sviluppare una partnership con il nostro laboratorio.   

Biografia 

2015 - oggi: Full Professor Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla CA.

2012 - oggi: Lab Director, Fondazione Santa Lucia, Roma, Italy.

2010 - oggi: Member of Editorial Board of Skeletal Muscle.

2002 - oggi: Member of Editorial Board of Journal of Cellular Physiology.

2001 - oggi: Member of Editorial Board of Cancer Biology and Therapy.

2012: Senior Telethon Scientist, Dulbecco Telethon Institute (DTI), Roma, Italy (position declined).

2010 - 2015: Associate Professor Sanford-Burnham Medical Research Institute.

2008 - 2014: Adjunct Professor of Pediatrics, UCSD, School of Medicine. La Jolla, CA.

2007 - 2012: Associate Telethon Scientist, Dulbecco Telethon Institute (DTI), Roma, Italy.

2004 - 2009: Assistant Professor, Burnham Institute for Medical Research, La Jolla, CA.

2002 - 2007: Assistant Telethon Scientist, Dulbecco Telethon Institute (DTI), Roma, Italy.

2002 - 2004: American Heart Association Beginning Grant-in-Aid.

2001 - 2004: Staff Scientist, Peptide Biology Laboratory, Salk Institute, La Jolla, CA.

2000 - 2003: MDA Development Grant.

1998 - 2000: Recipient of a Human Frontier Fellowship.

1997 - 2001: Postdoc, UCSD Department of Biology, La Jolla CA.

1992 - 1997: Recipient of a CEE Fellowship for a Postgraduate School of Internal Medicine.

1992 - 1997: Postgraduate & Clinical Internship Internal Medicine, University of Rome La Sapienza, Italy.

1986 - 1991: Medical Degree, Medicine and Surgery, University of Rome La Sapienza, Italy.

Incarichi Istituzionali 

2015 - oggi: Member, Duchenne Parent Project Spain.

2015 - oggi: Member, Stem Cell Genomics Core Advisory Board, University of California, San Diego.

2014: EMBO workshop, Molecular Biology of Muscle development and regeneration, Lecce – Italy.

2014: Stem Cell Meeting on the Mesa IX organizing committee.  2014. The Sanford Consortium for Regenerative Medicine.

2013: Sanford-Burnham Medical Research Institute, 35th Annual Symposium “Epigenetics: Development and Disease”, La Jolla, CA.

2009 - oggi: Member of the Advisory Board of Cure Duchenne.

2008 - 2011: Permanent Advisory Board member of the Parent Project/NIH/NINDS panel for EndDuchenne Prize.

 

HDAC-regulated myomiRs control BAF60 variant exchange and direct the functional phenotype of fibro-adipogenic progenitors in dystrophic muscles. Saccone V, Consalvi S, Giordani L, Mozzetta C, Barozzi I, Sandoná M, Ryan T, Rojas-Muñoz A, Madaro L, Fasanaro P, Borsellino G, De Bardi M, Frigè G, Termanini A, Sun X, Rossant J, Bruneau BG, Mercola M, Minucci S, Puri P.L., Genes & Development, 2014.

Fibroadipogenic progenitors mediate the ability of HDAC inhibitors to promote regeneration in dystrophic muscles of young, but not old mdx mice. Mozzetta C., Consalvi S., Saccone V., Tierney M., Diamantini A., Mitchel K.J., Marazzi G., Borsellino G., Battistini L., Sassoon D., Sacco A., Puri P.L. EMBO Mol. Med, 2013.

Epigenetic reprogramming of human embryonic stem cells (hESCs) into skeletal muscle cells and generation of contractile myospheres. Albini S., Coutinho P., Malecova B., Giordani L., Savchenko A., Forcales S, Puri P.L., Cell Rep, 2013.

Signal dependent incorporation of MyoD-BAF60c into Brg1-based SWI/SNF chromatin-remodeling complex. Forcales S., Albini S., Giordani L., Malecova B., Cignolo L., Chernov A., Coutinho P., Saccone V., Consalvi S., Williams R., Wang K., Wu Z., Baranovskaya S., Miller A., Dilworth F., Puri P.L., EMBO J, 2012.

TNF/p38 alpha/Polycomb signalling to Pax7 locus in satellite cells links inflammation to the epigenetic control of muscle regeneration. Palacios D., Mozzetta C., Consalvi S., Caretti G., Saccone V., Proserpio V., Marquez V.E., Valente S., Mai A., Forcales S., Sartorelli V., Puri P.L.,  Cell Stem Cell, 2010.

Functional interdependence at the chromatin level between the MKK6/p38 and IGF1/Pi3K/AKT pathways during muscle differentiation. Serra C., Palacios D., Mozzetta C Forcales S.,. Ripani M., Morantte I., Jones D. Du K., Jahla U., Simone C., Puri P.L., Mol Cell, 2007.

Functional and morphological recovery of dystrophic muscles in mice treated with deacetylase inhibitors. Minetti G. C., Colussi c., Adami R., Serra C.,  Mozzetta C., Parente V., Illi B., Fortuni S., Straino S., Gallinari P., Steinkhuler C., Capogrossi M., Sartorelli V., Bottinelli R., Gaetano C., Puri P.L., Nature Medicine, 2006.

p38 Pathway Targets SWI/SNF Chromatin Remodeling Complex to Muscle-Specific Loci. Simone C., Forcales S.V., Hill D., Imbalzano A.L., Latella L., and Puri P.L., Nature Genetics, 2004.

Stage-specific modulation of skeletal myogenesis by inhibitors of nuclear deacetylases. Iezzi S., Cossu G., Nervi C. Sartorelli V., and Puri P.L., Proc. Natl. Acad. Sci., 2002.

Class I histone deacetylases sequentially interact with MyoD and pRb during skeletal myogenesis. Puri P.L., Iezzi, S., Stiegler P., Chen T.T., Shiltz L., Muscat G., Giordano A, Wang J.Y.J. and Sartorelli V., Mol Cell, 2001.

Pediatrics (Stem Cells), UCSD, School of Medicine, La Jolla, California
Development and Regeneration course, SanfordBurnhamPrebys Medical Discovery Institute, PhD program